Genomic resources for Tor species are currently limited, and their molecular systematics remain poorly resolved. In this study, we report the first chromosome -level genome assembly of Tor sinensis, generated using PacBio long-read sequencing and Hi-C scaffolding. The final genome assembly spans approximately 1.80 Gb and consists of 228 contigs, with a contig N50 of 31.67 Mb. A total of 97 contigs were successfully anchored and ordered into 50 chromosomes, covering 96.57% of the genome. Chromosome lengths range from 23.67 Mb to 62.88 Mb. Repeat analysis identified 807.40 Mb of repetitive sequences, representing 44.84% of the genome. In addition, 3,794 rRNA genes and 11,264 tRNA genes were annotated, along with 379 snRNAs and 35 snoRNAs. The average length of tRNA genes was 72 bp. A total of 62 ,000 protein-coding genes were predicted, yielding 263,453 protein sequences. This high-quality reference genome provides a valuable foundation for future studies and facilitate comparative genomic analyses across the Tor genus and support biodiversity conservation efforts in the Lancang-Mekong River basin.

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